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Isolation of genomic DNA from whole blood
The goal of the phlebotomy visit is to collect 4 tubes of blood in the following order: . to 10cc blood each) Shipped to USC lab to be used for DNA extraction.
https://bioinformatics.dartmouth.edu/ccfrc/Downloads/Cfr-post_renewal_09092005.doc
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1 Selection and Preservation of whole blood and extraction of DNA a Take blood using blood collection tube with anticoagulants which is EDTA .
http://www.molecularstation.com/forum/dna-extraction-forum/73309-extraction-whole-blood-dna-rna.html | | -1 | Completely destroyed |
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Jan 30, 2012 . The facility offers genomic DNA extraction from whole blood, buffy coat, packed . If possible, collect blood in EDTA to reduce DNA degradation.
http://web.cgm.northwestern.edu/cgm/Core-Facilities/Genomics-Core/Genomic-Services/DNA-Extraction |
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Jan 18, 2007 . DNA extraction from the fragmented blood clot mass obtained from the . to extract DNA from the blood clot as soon as possible after collection.
http://www.clinchem.org/content/53/3/522.full |
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Effects of blood storage time and temperature on DNA yield and ...
Therefore, the effects of storage time (number of days from blood collection to DNA extraction) and temperature on DNA yield and quality are important.
http://www.ncbi.nlm.nih.gov/pubmed/8431281 Genomic DNA extraction from whole blood stored from 15- to 30 ...
Oct 26, 2010 . Long-term stored (LTS) whole blood collection can be an important . DNA extraction methods using LTS -20 °C whole blood from 1980, and .
http://www.ncbi.nlm.nih.gov/pubmed/21029772
Aspergillus DNA contamination in blood collection tubes
ination originating from blood collection tubes and a variety of other clinically relevant vessels from a range of manufacturers using a DNA extraction kit that has .
http://www.aspergillus.org.uk/pdfs/20638611.pdf Document Libraries | Molecular Medicine Ireland
The Guidelines include standardised procedures on blood, buccal cells, . for DNA extraction.pdf; SOP 2.05.04 Blood Collection for RNA isolation.pdf; SOP .
http://www.molecularmedicineireland.ie/libraries/libgroup/8 NUCLEON GENOMIC DNA EXTRACTION KIT
BLOOD COLLECTION. Blood collected in potassium EDTA tubes is suitable for genomic DNA extraction using this kit. Heparinised or citrated blood is also .
http://www.gen-probe.com/pdfs/downloads/protocol%20BACC%20123[1].pdf
PreAnalytiX - PAXgene Blood DNA Kit
The system requires the combined use of PAXgene Blood DNA Tubes for blood collection and stabilization, followed by DNA isolation using the PAXgene Blood .
http://www.preanalytix.com/product-catalog/blood/dna/products/paxgene-blood-dna-kit/
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Isolation of DNA from Dried Blood Spot Using the High Pure PCR ...
Freezing anticoagulated blood samples might interfere with the efficiency of DNA isolation afterwards. Thus, the collection and storage of whole blood samples .
http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/no4_09/pdfs/15.pdf
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The quality of DNA extracted from liquid or dried blood is not ...
Feb 8, 2008 . Methods Fresh blood samples (n = 80) were collected and either . was to compare the suitability of DNA extracted from blood collection cards .
http://ije.oxfordjournals.org/content/37/suppl_1/i7.full
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BMC Medical Genetics | Full text | Satisfaction survey with DNA ...
Blood samples obtained by venepuncture (the standard DNA collection method) . High-quality genomic DNA can be extracted from these samples and used for .
http://www.biomedcentral.com/1471-2350/7/45
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Product Circular PAXgene Blood DNA Tube
PAXgene™ Blood DNA Tube. FOR THE COLLECTION OF WHOLE BLOOD. FOR GENOMIC DNA ISOLATION. For Research Use Only. Not for use in diagnostic .
http://www.qiagen.com/hb/paxgeneblooddnatube A Simple Automated DNA Extraction Method for Dried Blood ...
We developed a simple, inexpensive, and automated procedure for the extraction of genomic DNA from dried blood specimens (DBS) collected on filter paper.
http://jla.sagepub.com/content/10/5/310.full
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DNA extraction from saliva samples collected with the Oragene ...
Oragene®•DNA Self-Collection kit are stable for years at room temperature and expensive shipment cost associated with blood collection is therefore avoided.
http://www.dnagenotek.com/US/pdf/568446855.pdf Genomic DNA Isolation from Human Whole Blood
Collection tubes. (CT). • Caps. (CAP). • Waste tubes. (WT). Results : Genomic DNA isolation from Human Whole Blood. ? AGE of isolated genomic DNA .
http://www.autogen.com/assets/pdf/Application%20Guide%20No.02-DNA%20Whole%20Blood.pdf
KAPABIOSYSTEMS
of DNA extracts. The KAPA Express Extract enzyme and buffer are combined in a single tube with EDTA blood (2 – 8 ?l), or a punch from a blood collection card .
http://www.kapabiosystems.com/public/pdfs/kapa-express-extract-kits/KAPA_Express_Extract_Blood_PCR_Note.pdf
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Biobanking and Databasing - The Centre for Applied Genomics
For DNA extraction from blood, collection of the blood sample in lavender top ( EDTA) tubes is recommended. For establishment of cell lines, collection of the .
http://www.tcag.ca/facilities/biobanking.html
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Problem with anticoagulant for blood collection - Molecular Biology
Apr 13, 2007 . i collect blood from retro orbital site from rat using capillary filled with EDTA and . i want to prepare DNA extraction from blood ready for PCR.
http://www.protocol-online.org/biology-forums/posts/26327.html
AquaPreserve - www.aquaplasmid.com
It may be used to streamline blood sample collection, stabilization, transport, storage, and DNA, RNA, and protein extraction. The extraction protocols are simple.
http://www.aquaplasmid.com/AquaPreserve.html
Aspergillus DNA contamination in blood collection ... - ScienceDirect
Up to 18% of blood collection tubes were contaminated with fungal DNA, probably . DNA extraction was performed in a laboratory that is environmentally .
http://www.sciencedirect.com/science/article/pii/S0732889310000842
Laboratory Disposable Products - Medical Laboratory Supplies and ...
Disposable Non Sterile Specimen Collection Containers · Disposable Sterile . Vayugen DNA Extraction Kit · Genomic DNA . Biopro Blood Collection Tubes .
http://www.bioproindia.com/
Isohelix an Integrated DNA strategy. DNA Buccal Swabs DNA ...
quality buccal cell and genomic DNA. Buccal cell sampling offers a viable alternative to blood collection and, together with the Isohelix range of. DNA Isolation .
http://www.isohelix.com/PDF/isohelix_swab_brochure.pdf
NucleoSpin Dx Blood NucleoSpin Dx Blood
Suitable for EDTA, citrate, and heparin blood collecting systems. For fresh and frozen blood samples. Highly reliable DNA isolation from whole blood .
http://www.biolynx.ca/literature/mcn_nsdxblood.pdf
DNA Extraction from FTA® Cards Using the GenSolve DNA ...
FTA® Technology is a fast and easy way to collect DNA from a variety of biological samples for rapid DNA preparation and for room temperature storage. Blood .
http://www.whatman.com/References/GenSolveDataSellSheetLR.pdf
Georgia Genomics Facility | Sample Prep
Repeated freezing and thawing of stored samples reduces DNA size. Fresh blood collected in EDTA tubes. Qiagen blood DNA extraction kits (QIAamp DNA .
http://dna.uga.edu/dna-extraction-and-purification/sample-prep/
DNA extraction from filter spots with dried blood
Application Note. DNA extraction from filter spots with dried blood. Introduction. For blood collection and storage the use of filter papers is an attractive alternative .
http://www.molegenetics.com/sites/m/molegenetics.com/files/5cea52eb1af5c1067883da2195c36122.pdf
Standard Operating Procedure (SOP) 002 V 2.0
collection event. Blood cells are stored at -800C until ready for DNA extraction. Extraction: DNA is extracted from the blood cells at the Indiana CTSI Specimen .
http://komentissuebank.iu.edu/download/18
PreAnalytiX - PAXgene Blood DNA Tube
The system requires the combined use of PAXgene Blood DNA Tubes for blood collection and stabilization, followed by DNA isolation using the PAXgene Blood .
http://www.preanalytix.com/product-catalog/blood/dna/products/paxgene-blood-dna-tube/
DNA Preparation Techniques - Office of Research
Two routes of blood collection that do not require anesthesia are the saphenous vein and . A variety of kits are available for extracting DNA from mouse tissue.
http://www.research.uci.edu/tmf/DNAprep.htm
MagDEA® DNA Whole Blood (8Lx) | Product Lineup | Products ...
Direct extraction and purification of genomic DNA (without pretreatment) through blood collecting tube. The elution volume is 1 ml. High purified DNA allowing .
http://www.pss.co.jp/english/product/product/details-reagent-09.html
DNAgard Blood Tube preserves DNA in whole blood | Biomatrica ...
DNAgard® Blood Tube is designed for the collection, preservation and shipping of whole blood samples for genomic DNA isolation. The preservative reagent .
http://www.biomatrica.com/dnagardblood_tube.php
Nonenzymatlc Extraction of DNA from Blood Collected
Nonenzymatlc Extraction of DNA from Blood Collected into Serum Separator Tubes, Nikolaos Siafakas,'. Leslie. Burnett,1'3 Bruce Bennetts,2 and Anne Proos' .
http://www.clinchem.org/content/41/7/1045.full.pdf
Low sensitivity of nested PCR using PlasmodiumDNA extracted from
A nested PCR protocol with DNA extracted from two blood storage devices obtained . At the time of blood collection (1996) these individuals were living in the .
http://malariajournal.com/content/3/1/8
DNA isolation kits - Products, news, whitepaper, companies ...
Freezing anticoagulated blood samples might interfere with the efficiency of DNA isolation afterwards. Thus, the collection and storage of whole blood samples .
http://www.bionity.com/en/dna-isolation-kits.html
High-Concentration Genomic DNA From Whole Blood Using the ...
In this article we describe a novel DNA isolation chemistry with an automated . Whole blood from a single donor was collected into Vacutainer® tubes (BD .
http://www.promega.com/resources/articles/pubhub/high-concentration-genomic-dna-from-whole-blood-using-the-maxwell-16-low-elution-volume-instrument/
Isohelix an Integrated DNA strategy. DNA Buccal Swabs DNA ...
quality buccal cell and genomic DNA. Buccal cell sampling offers a viable alternative to blood collection and together with the Isohelix range of. DNA Isolation .
http://www.cellprojects.com/PDF/isohelix_swab_brochure.pdf
Comparison of IsoCode STIX and FTA Gene Guard Collection ...
DNA was extracted from the blood samples collected by the three methods. Genomic DNA was extracted from the frozen whole-blood samples with a QIAamp .
http://jcm.asm.org/content/39/3/1195.full
Collecting and processing buccal cell samples
Blood collection and deposition onto membranes . The Buccal DNA Collector, BodeXtract DNA Extraction Reagent, and BodeElute. DNA Extraction Reagent .
http://www.isfg.org/files/dc16f182fbefb9ba18c36aff32e06c8f08fabcfa.03014857_283754646293.pdf
The Use of Filter Paper to Collect Blood Samples for Diagnostic ...
Perhaps an alternative matrix would have properties more suitable for DNA extraction. One approach to making a blood collection paper that allowed for simpler .
http://www.acefesa.es/bio/fta/903/documentos/903.doc
DNA Isolation from Fresh and Frozen Blood, Tissue Culture Cells ...
Aug 27, 2004 . Blood Volumes and Incubation Times for Animal DNA Isolation . 2-10. Purifying DNA from Blood on ABI PRISM™ Sample Prep Stations . . 2-12 .
http://docs.appliedbiosystems.com/pebiodocs/04343586.pdf
Isolation of Genomic DNA from Mammalian Cells
The collection and isolation of DNA from blood samples has several disadvantages. Typically blood collection and subsequent DNA isolation are uncomfortable, .
http://onlinelibrary.wiley.com/doi/10.1002/0471142905.hga03bs19/pdf
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UltraClean™ Blood DNA Isolation Kit
The UltraClean™ Blood DNA Isolation Kit is designed to isolate high molecular weight genomic . 15 ml Collection Tubes; MO BIO catalog#12700-T (bag of 25) .
http://www.mobio.com/images/custom/file/12000%20&%2012002-1000%20(booklet)(1).pdf
SHORT REPORT: RAPID DNA EXTRACTION FROM ARCHIVE ...
Collection of blood samples on filter paper has a history of more than 40 years.1 . An optimal DNA isolation process is therefore a pre-requisite for quality and .
http://www.ajtmh.org/content/72/3/249.full
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